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Comparison of pTAL4 and pTAL7 vector systems

Journal: BMC Genomics

Article Title: A modified TALEN-based system for robust generation of knock-out human pluripotent stem cell lines and disease models

doi: 10.1186/1471-2164-14-773

Figure Lengend Snippet: Comparison of pTAL4 and pTAL7 vector systems

Article Snippet: TALENs were assembled as published [ ], by following the protocol associated with the GoldenGate TALEN and TAL Effector Kit 1.0 (Addgene #1000000016), except that TALE repeats were ultimately cloned into vectors pTAL7A (for TALEN A) and pTAL7B (for TALEN B) (Additional file : Figure S1).

Techniques: Plasmid Preparation, Clone Assay, Expressing, Sequencing, Transfection, FLAG-tag